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http://nopr.niscpr.res.in/handle/123456789/10102| Title: | Comparative evaluation of PCR in Ziehl-Neelsen stained smears and PCR in tissues for diagnosis of Mycobacterium avium subsp. paratuberculosis |
| Authors: | Coelho, A C Pinto, M L Miranda, A Coelho, A M Pires, M A Matos, M |
| Keywords: | Mycobacterium avium subsp. paratuberculosis;PCR;tissues;Ziehl-Neelsen |
| Issue Date: | Sep-2010 |
| Publisher: | CSIR |
| Abstract: | Thirty six tissues from sheep, previously diagnosed with paratuberculosis, were tested by PCR in positive Ziehl-Neelsen staining smears of tissues, and PCR in tissues targeting IS900 specific for Mycobacterium avium subsp. paratuberculosis. DNA amplification was achieved in 33.3% Ziehl-Neelsen smears, and in 61.1% tissue samples. Combination of both techniques found 66.7% samples as positive. Combination of techniques would, therefore, increase the sensitivity of diagnosis. |
| Page(s): | 948-950 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Appears in Collections: | IJEB Vol.48(09) [September 2010] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJEB 48(9) 948-950.pdf | 62.9 kB | Adobe PDF | View/Open |
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