Growth of 3T3 fibroblast on collagen immobilized poly(ethylene terephthalate) fabric

Abstract

Radiation induced grafting of acrylic acid (AA) and binary mixture of acrylic acid/<i style="">N</i>-vinyl pyrrolidone (NVP) has been carried out on poly(ethylene terephthalate) (PET) fabric. The grafted fabrics are immobilized with collagen via carbodiimide coupling to make the fabric bioreceptive and biocompatible for cell seeding and grafts. Atomic force microscopy and scanning electron microscopy observations suggest that the collagen has a very similar structure on PET-<i style="">g</i>-AA/NVP and PET-<i style="">g</i>-AA. After immobilizing collagen, PET induces growth and proliferation of 3T3 mouse fibroblasts as compared to virgin PET. The results indicate that collagen immobilized PET-<i style="">g</i>-AA fabric shows better adhesion and proliferation than PET-<i style="">g</i>-AA/NVP fabric.