Studies on DNA fingerprinting in Murrah buffaloes using microsatellite markers

Abstract

For the present study, blood samples from 35 Murrah dam-progeny pairs and semen samples of their sires were collected. All six primers successfully amplified the genomic DNA from all the animals. The mean observed heterozygosity (H_o) within the population was found to be 0.5438 and it ranged from 0.375 (CSSM43) to 0.775 (CSSM61). Average expected Levene’s and Nei’s heterozygosity (H_ep) were 0.6209 ±0.0894 and 0.6170 ±0.0889, ranging from 0.5155 to 0.7575 and 0.5123 to 0.7528, for loci CSSM43 and CSSM61, respectively. Both observed and expected heterozygosity was above 0.5, which shows that there is sufficient variability in the population and reflects presence of large number of polymorphic loci in the breed. The mean PIC value was 0.5661, ranging from 0.476 to 0.718, for the loci CSSM43 and CSSM61, respectively. In the present study, the probability of exclusion (PE) ranged from 0.0994 to 0.5535 for the loci CSSM43 and CSSM61, respectively. The combined PE (PEc) for all 6 loci was 0.845. Based on PE_c, it was shown that DNA analysis in the examined herd of Murrah buffaloes allows incorrect parentage to be excluded with 84.5% probability.