Standardization of DNA isolation and PCR parameters in <i style="">Garcinia </i>spp. for RAPD analysis

Asish, G R; Parthasarathy, Utpala; Nithya, N G

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Please use this identifier to cite or link to this item: http://nopr.niscpr.res.in/handle/123456789/10443

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DC Field	Value	Language
dc.contributor.author	Asish, G R	-
dc.contributor.author	Parthasarathy, Utpala	-
dc.contributor.author	Nithya, N G	-
dc.date.accessioned	2010-10-13T08:26:42Z	-
dc.date.available	2010-10-13T08:26:42Z	-
dc.date.issued	2010-10	-
dc.identifier.issn	0975-0967 (Online); 0972-5849 (Print)	-
dc.identifier.uri	http://hdl.handle.net/123456789/10443	-
dc.description	424-426	en_US
dc.description.abstract	Doyle and Doyle protocol with a little modification was used for the isolation of DNA from leaves and fruit rind of Garcinia spp. In case of leaves and dry fruit rind, the best DNA isolation was possible with 4% CTAB (100 mM Tris, 30 mM EDTA & 1.4 M NaCl), followed by 1.5% PVP and 0.3% mercaptoethanol. In case of fresh fruit rind, the DNA was best isolated with 2% CTAB (100 mM Tris, 30 mM EDTA & 1.4 M NaCl). PCR parameters were optimized using random RAPD primers. Other parameters included the DNA concentration at 50 ng/reaction, annealing temperature of 43°C, 2.5 mM dNTP in presence of 1 U Taq DNA polymerase and 2.0 mM MgCl₂. The protocol will be of great help to study the genetic diversity of Garcinia germplasms.	en_US
dc.language.iso	en_US	en_US
dc.publisher	NISCAIR-CSIR, India	en_US
dc.rights	CC Attribution-Noncommercial-No Derivative Works 2.5 India	en_US
dc.source	IJBT Vol.9(4) [October 2010]	en_US
dc.subject	DNA extraction	en_US
dc.subject	Garcinia	en_US
dc.subject	fresh fruit rind	en_US
dc.subject	dry fruit rind, RAPD-PCR	en_US
dc.title	Standardization of DNA isolation and PCR parameters in Garcinia spp. for RAPD analysis	en_US
dc.type	Article	en_US
Appears in Collections:	IJBT Vol.09(4) [October 2010]

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