Antioxidant potential of n-butanol fraction from extract<i style=""> </i>of<i style=""> Jasminum mesnyi</i> Hance leaves

Abstract

Methanolic extract of <i style="">Jasminum mesnyi </i>Hance leaves having antidiabetic activity was subjected to fractionation to obtain antioxidant and antihyperglycemic rich fraction. Different concentrations of ethyl acetate and n-butanol fractions were subjected to antioxidant assay by DPPH method, nitric oxide scavenging activity and reducing power assay. The fractions showed dose dependent free radical scavenging property in all the models. IC₅₀ values for ethyl acetate and n-butanol fractions were 153.45±6.65 and 6.22±0.25 <img src='/image/spc_char/micro.gif' border=0> g/ml, respectively, as compared to L-ascorbic acid and rutin (as standards; IC₅₀values 6.54±0.24 and 5.43±0.21 <img src='/image/spc_char/micro.gif' border=0> g/ml, respectively) in DPPH model. In nitric oxide scavenging activity, IC₅₀ values were 141.54±9.95 <img src='/image/spc_char/micro.gif' border=0> g/ml, 35.12±1.58 <img src='/image/spc_char/micro.gif' border=0> g/ml, 21.06±0.95 <img src='/image/spc_char/micro.gif' border=0> g/ml and 29.93±0.32 <img src='/image/spc_char/micro.gif' border=0> g/ml for ethyl acetate, n-butanol fractions, L-ascorbic acid and rutin, respectively. n-Butanol fraction showed a good reducing potential and better free radical scavenging activity as compared to ethyl acetate fraction. Potent antioxidant n-butanol fraction showed better oral glucose tolerance test (antihyperglycemic) at par with metformin (standard drug). n-Butanol fraction contained secoiridoid glycosides which might be responsible for both antioxidant and antihyperglycemic activity.