Inhibition of UV induced adversaries by <img src='/image/spc_char/beta.gif' border=0>-glucogallin from Amla (<i>Emblica officinalis</i> Gaertn.) fruits

Abstract

Photoprotection efficacy of Amla (<i style="">Emblica officinalis</i> Gaertn.) fruit extract was investigated by various <i>in vitro</i> models. It was found that 1-O-Galloyl-<img src='/image/spc_char/beta.gif' border=0>-D-glucose (<img src='/image/spc_char/beta.gif' border=0>-glucogallin) active in Amla extract has a significant role in photoprotection efficacy due to its inhibitory effect on Ultraviolet irradiation (UV) induced adversaries in cell cultures. <img src='/image/spc_char/beta.gif' border=0>-Glucogallin treatment at a concentration of 10 to 80 µg/ml in B16F1 mouse melanoma cells showed a dose dependent decrease of 15 to 55% in UV induced melanogenesis. <img src='/image/spc_char/beta.gif' border=0>-Glucogallin treatment at a concentration of 10 to 80 µg/ml, also showed a dose dependent decrease of 30 to 90% in UV induced cytotoxicity and a decrease of 42 to 82% in UV induced Reactive Oxygen Species (ROS) generation in Swiss 3T3 mouse fibroblast cells. It showed a very high Oxygen Radical Absorbance Capacity (ORAC) value of 4200 µmol Trolox equivalents/g. Our evaluations shown that <img src='/image/spc_char/beta.gif' border=0>-glucogallin from Amla extract has significant photoprotection efficacy.