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Title: Rapid and efficient plant regeneration of eggplant (Solanum melongena L.) from cotyledonary leaf explants
Authors: Shivaraj, G
Rao, Srinath
Keywords: Brinjal;cotyledonary leaf;eggplant;plant regeneration;IBA;Solanum melongena
Issue Date: Jan-2011
Publisher: NISCAIR-CSIR, India
Abstract: The aim of this study was to develop an efficient protocol for establishment of plant regeneration through cotyledonary leaf explants of Eggplant (Solanum melongena L.). Two varieties of brinjal [Pusa purple long (PPL) and Black beauty (BB)] were used. High frequency and rapid regeneration protocol was developed from cotyledonary leaf explants on MS medium supplemented with either 6-Benzyl amino purine (BAP), Kinetin (Kn), Thidiazuron (TDZ) or Zeatin (Ze). The highest number of shoots (23.3±0.10) was obtained on MS medium containg 2.0 mg/BAP+0.5 mg/L Kn. The in vitro regenerated small shoots were further elongated on MS medium supplemented with gibberellic acid (GA3) at 1.5 mg/L. Elongated shoots were then excised from shoot clumps and transferred to rooting medium containg indole butyric acid (IBA) at 3.0 mg/L. The rooted plantlets were hardened on MS basal liquid medium and subsequently transferred to polycups containg vermiculate:soil:sand (1:2:2). Plantlets, thus developed were successfully established and finally transferred to a greenhouse. The plantlets showed high survival rate (80%) in the soil.
Page(s): 125-129
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.10(1) [January 2011]

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