Low copy number DNA typing of touched evidence by miniSTRs—A case study

Abstract

Low copy number (LCN) DNA typing is an improvised technique to obtain genetic profile from sub-optimal quantity of DNA recovered from the biological evidence samples. Minute quantity of DNA available from the touched materials sometimes fail to generate DNA profile employing conventional STR multiplexes. STRs with reduced amplicon size are a better tool for generating profile from the samples with sub-optimal quantity of DNA or environmentally challenged. In the present case study, DNA profiling of touched evidence materials is reported employing a combination of LCN typing and miniSTRs. The technology is highly valuable for increasing the scope of DNA profiling to a large number of touched evidence materials.