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http://nopr.niscpr.res.in/handle/123456789/11302| Title: | Detection of Equine Influenza Viral Genome by RT-PCR and RNA-DNA Hybridization |
| Authors: | Gupta, A K Ahlawat, Anita Bhatia, Vandanajay |
| Keywords: | Equine influenza;RT-PCR;Nucleic acid hybridization |
| Issue Date: | Apr-2003 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Reverse transcriptase-polymerase chain reaction (RT-PCR) and RNA : DNA hybridization based diagnostic techniques were standardized and used for rapid and specific detection of equine influenza virus. RT -PCR technique based on amplification of both Non-structural-l (NS1) and Neuraminidase (NA) genes was standardized. Beside this, RNA-DNA hybridization technique using both non-structural and neuraminidase gene segments specific hot probes was also developed to directly detect this virus with out involving viral RNA isolation. Both RT-PCR and RNA-DNA hybridization techniques were successfully used for detection of influenza virus in samples within 24 to 48 hrs. These techniques can now be used as a routine tests in the laboratory for detection of influenza virus from field samples. |
| Page(s): | 214-219 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Appears in Collections: | IJBT Vol.02(2) [April 2003] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJBT 2(2) 214-219.pdf | 1.3 MB | Adobe PDF | View/Open |
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