Evaluation of Yeast as an Expression System

Abstract

Developments in recombinant DNA technology have provided an alternate route for the production of proteins.

Gene expression and production of proteins of interest are of great importance for basic research as well as for

biomedical applications. A number of expression systems using mammalian cells, insect cells, yeast and other

bacteria as host have been developed. Yeast has received attention as a suitable host for expression of many

mammalian genes due to many specific characteristics. Yeast strains, <i>Schizosaccharomyces pombe, Saccharomyces</i>

<i>cerevisiae </i>and <i>Pichia pastoris, </i>have many advantages over other systems and may be the host of choice for the

expression of complex proteins of therapeutic value. During the post-genomicera, the importance of these strains for the expression of heterologous genesmay enhance considerably.