Isolation and characterization of 37 kDa heparinase from the purple fluid of <i style="">Dolabella auricularia</i>

Abstract

Present study was to explore the heparinase and antiglycosidase activity of the purple fluid of <i style="">Dolabella auricularia</i>. Heparinase activity was found as 56.42 IU/ml, further the activity was confirmed by APPT test by the reduction of coagulation time with the addition of purple fluid of about 7±0.2. This purple fluid was dialyzed and purified by anion exchange column (DEAE-cellulose). Amount of protein in crude and purified sample was 2mg/ml and 7.6mg/ml. Apparent molecular weight of the purified heparinase was reveled by SDS-PAGE as 37 kDa. Effects of various factors such as temperature and pH were determined and recorded the maximum activity at 30ºC-50ºC and 7 respectively. Antibacterial activity was also observed against four pathogens and activity was observed maximum only in <i style="">P. aeruginosa</i>, <i style="">S. aureus</i>. Antiglycosidase activity exhibits about 120.9IU/ml which has been reduced when compared to that standard. Present study suggests the purple fluid of <i style="">D. auricularia</i> has immense uses in pharmaceutical industries.