Enhanced production of alpha amylase using vegetables wastes by <i style="">Aspergilllus niger</i> strain SK01 marine isola

Abstract

155 Fungal isolates, isolated from marine water, belong to five fungal species viz <i style="">Aspergillus niger, A. fumigatus, Penicillium expansum, Trichoderma virens </i>and<i style=""> Penicillium </i>spp were screened for alpha amylase production. All the isolates belong to <i style="">A.niger</i>, 12 isolates of <i style="">Trichoderma virens </i>and <i style="">A.fumigatus</i>, 10 isolates of <i style="">Penicillium expansum</i> and 2 isolates of <i style="">Penicillium </i>spp , were produced alpha amylase. <i>A.niger </i>strain SK01 was selected for alpha amylase production. Vegetables wastes were used as a substrate by solid state fermentation under 0,10,20,40 and 50% moisture content. Enzyme activity was studied at 30°C, 40°C, 50°C, 60°C, 70°C temperature and the pH 5,6,7,8 and 9. Wastes supported growth and enzyme activity at all parameters tested. Maximum activity was recorded at 0% moisture content, 70°C temperature and pH 9.