Establishment of a rapid multiplication protocol of <i>Coleus forskohlii</i> Briq. and <i>in vitro</i> conservation by reduced growth

Abstract

<i style="">Coleus</i> <i style="">forskohlli </i>Briq., an endangered medicinal plant, has been successfully micropropagated under <i style="">in vitro</i> conditions. Best adventitious shoots were developed in solid Murashige and Skoog’s medium supplemented with 1.5 mg L^-l 6-benzylaminopurine, while best rhizogenesis was achieved without growth regulator. The second event of success was <i>in vitro</i> conservation of the species by slow growth technique employing osmotic regulators (sorbitol and mannitol). 3 <i style="">M</i> mannitol showed best performance for reducing growth. This study elucidated an efficient method of <i>ex situ</i> conservation of germplasm and sustainable management of this medicinally important plant.