Kappa casein gene polymorphism in Zalawadi goats

Abstract

Kappa casein protein variants were analysed in blood samples of 50 unrelated Zalawadi goat breeds by PCR-RFLP techniques with <i>Hae</i>III restriction enzyme. Two primers (5΄-TCC CAA TGT TGT ACT TTC TTA ACA TC-3΄) and (5΄-GCG TTG TCC TCT TTG ATG TCT CCT TAG-3΄) were used as forward and reverse primers, respectively for the amplification of <i style="">CSN3</i> gene. The resultant PCR product observed at 645 bp was digested with restriction endonuclease <i>Hae</i>III, and the resultant fragments of 416 bp and 229 bp after separation by electrophoresis were visualized and documented by gel documentation system, which indicated the presence of Kappa casein AA genotype in population. The band sizes were compared with molecular size marker and found to be of the same size in all the 50 samples.