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http://nopr.niscpr.res.in/handle/123456789/11456| Title: | Micropropagation and conservation of rare medicinal plant Wattakaka volubilis (Linn.) Stapf |
| Authors: | Arulanandam, L John Peter Kumar, S Ghanthi Sowmini, M |
| Keywords: | Wattakaka volubilis;Medicinal plant;Shoot segments;Mass propagation |
| Issue Date: | Apr-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | A micropropagation protocol for the important medicinal plant species Wattakaka volubilis was developed using shoot tip and nodal explants by culturing on Murashige-Skoog (MS) media fortified with 1.5 mg/L BAP in combination with 0.7 mg/L Kn that resulted in 5.3±1.4 multiple shoots per explant. Maximum shoot length observed was 5.8±1.1 cm on media supplemented with 1.5 mg/L BAP and 0.6 mg/L Kn. The in vitro raised micro shoots produced highest percentage of rooting (85%) in MS media augmented with 1.0 mg/L IBA. The hardened plantlets were acclimatized in the green house conditions and then reintroduced in the Herbal garden attached to the Centre in which 76% of the plants survived. |
| Page(s): | 238-241 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Appears in Collections: | IJBT Vol.10(2) [April 2011] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJBT 10(2) 238-241.pdf | 110.27 kB | Adobe PDF | View/Open |
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