Immobilization of peroxidase on a cost free support from salt fractionated proteins of bitter gourd

Abstract

This study presents direct immobilization of peroxidases from ammonium sulphate fractionated proteins of bitter gourd on cost free support, fly ash. Activated fly ash (<img src='/image/spc_char/Etta.gif' border=0>, 0.84) was quite effective in high yield immobilization of peroxidase from bitter gourd and it bind nearly 1113 enzyme units per g of support in sodium acetate buffer, pH 5.0. This enzyme bound very strongly on this support and it did not detach even in the presence of 1.0M NaCl. Fly ash adsorbed bitter gourd peroxidase retained 50% activity after treatment with 2.5 mg trypsin/ml of incubation mixture for 1 h at 40°C while soluble enzyme lost nearly 65% of initial activity under similar incubation conditions. FT-IR spectra of fly ash and adsorbed peroxidase showed different peaks at respective wavenumbers.