Identification and genetic purity testing of cotton F₁ hybrid using molecular markers

Abstract

<i style="">Gossypium hirsutum</i> F₁ hybrid Phule-388 and it’s parents RHCb-001 as male and RHC-006 as female were studied for identification and genetic purity testing with three PCR based molecular markers. Twenty RAPD primers, 19 ISSR primers and 33 cotton microsatellite loci were used for the analysis. RAPD primers, OPA-08 and OPA-11, were found to be useful in differentiating parents and hybrid. Two ISSR primers, IS-10 and IS-12, were identified. Two male specific amplicons of 1200 bp, 1500 bp and 1000 bp, 1300 bp each were also present in the hybrid. Microsatellite loci, JESPR-151 and JESPR-152 were found to be heteroallelic for parents. JESPR-151 identified male specific 140 bp repeat and female specific 180 bp repeat while JESPR-152 detected one male and female specific repeats of 120 bp and 180 bp, respectively. Results indicated that using all the three markers-RAPD, ISSRs and Microsatellites in combination is more reliable than using the three in isolation for identification and testing of genetic purity of cotton hybrids.