Please use this identifier to cite or link to this item:
http://nopr.niscpr.res.in/handle/123456789/1252| Title: | Ion pairing RP-HPLC analytical methods for simultaneous estimation of simvastatin and its â-hydroxy acid |
| Authors: | Madan, Jitender Thakkar, Vikrant Dwivedi, Anil Kumar Singh, Satyawan |
| Keywords: | HPLC assay method;Hypolipidemic agent;Simvastatin |
| Issue Date: | May-2007 |
| Publisher: | CSIR |
| IPC Code: | A61K |
| Abstract: | Sensitive HPLC assay methods for Simvastatin (SV), a hypolipidemic agent, and its corresponding â-hydroxy acid (SVA), were developed and validated for their simultaneous estimation in solutions of various studies. HPLC separations were achieved on (i) C₈ Lichrocart® Lichrosphere® (ii) CN Lichrocart® Lichrosphere® and (iii) C₁₈ Phenomenex® columns. The eluents were monitored by diode array detector at 240 nm. Retention times were: SV, 8-9; and SVA, 5.5-6 min. The lower limit of detection of both SV and SVA on C₁₈ column was 0.05 μg/ml and on C₈ and CN columns was 0.1 μg/ml. Reproducibility of the method were determined by inter and intra assay precision (< 6%). |
| Page(s): | 371-376 |
| ISSN: | 0022-4456 |
| Appears in Collections: | JSIR Vol.66(05) [May 2007] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| JSIR 66(5) (2007) 371-376.pdf | 111.03 kB | Adobe PDF | View/Open |
Items in NOPR are protected by copyright, with all rights reserved, unless otherwise indicated.