Please use this identifier to cite or link to this item:
Full metadata record
DC FieldValueLanguage
dc.contributor.authorTomar, Neha R-
dc.contributor.authorChandra, Rajesh-
dc.contributor.authorkumar, Rajiv-
dc.contributor.authorTiwari, A K-
dc.contributor.authorKumar, Anil-
dc.identifier.issn0975-1009 (Online); 0019-5189 (Print)-
dc.description.abstractThe present study was undertaken to clone, express rabies virus glycoprotein (RVG) and to identify potential T-cell epitopes on it. RVG gene (1590 bp) was amplified using gene specific primers. The amplified product was cloned into pTZ57R/T cloning vector by TA cloning. RVG gene was subcloned into pcDNA3.1 (+) expression vector. In this study, cloning and expression of rabies virus glycoprotein gene was done under CMV promoter and an expression construct (pcDNA.RVG) was prepared and clones were confirmed by restriction digestion, colony PCR and nucleotide sequencing. The expression construct was further characterized by western blotting and indirect fluorescent antibody test (IFAT). In silico analysis of this protein was done to find out potential antigenic sites so that it can be further evaluated for its potential as candidate for epitope vaccine against rabies.en_US
dc.publisherNISCAIR-CSIR, Indiaen_US
dc.rights CC Attribution-Noncommercial-No Derivative Works 2.5 Indiaen_US
dc.sourceIJEB Vol.49(08) [August 2011]en_US
dc.subjectDNA vaccineen_US
dc.subjectEpitope mappingen_US
dc.subjectIndirect fluorescent antibody testen_US
dc.subjectRabies virusen_US
dc.titleExpression of rabies virus glycoprotein gene into eukaryotic system and determination of potential T-cell epitopesen_US
Appears in Collections:IJEB Vol.49(08) [August 2011]

Files in This Item:
File Description SizeFormat 
IJEB 49(8) 594-599.pdf375.63 kBAdobe PDFView/Open

Items in NOPR are protected by copyright, with all rights reserved, unless otherwise indicated.