Protoplast formation and regeneration of Acetobacter pasteurianum NCIM 2314

Singhvi, Mamta; Joshi, Dipti; Gokhale, D V

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Please use this identifier to cite or link to this item: http://nopr.niscpr.res.in/handle/123456789/12584

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DC Field	Value	Language
dc.contributor.author	Singhvi, Mamta	-
dc.contributor.author	Joshi, Dipti	-
dc.contributor.author	Gokhale, D V	-
dc.date.accessioned	2011-08-16T07:03:46Z	-
dc.date.available	2011-08-16T07:03:46Z	-
dc.date.issued	2011-06	-
dc.identifier.issn	0975-2412 (Online); 0771-7706 (Print)	-
dc.identifier.uri	http://hdl.handle.net/123456789/12584	-
dc.description	94-96	en_US
dc.description.abstract	Method for production and regeneration of Acetobacter pasteurianum NCIM 2314 protoplasts are described. The protoplasts were obtained by treatment with lysozyme in protoplast buffer at pH 8.0 with different osmotic stabilizers. The protoplasts were regenerated on Acetobacter medium with various osmotic stabilizers. Maximum protoplasts formation was obtained in protoplasts buffer with NaC1 as an osmotic stabilizer using lysozyme (0.5mg/mL). Maximum protoplast regeneation was obtained on Acetobacter medium with NaCl (0.5M) as an osmotic stabilizer. The regeneration medium was also applicable to other species of Acetobactor as well. This is, to our knowledge, the first report on protoplast formation and efficient regeneration in case of Acetobacter pasteurianum.	en_US
dc.language.iso	en_US	en_US
dc.publisher	NISCAIR-CSIR, India	en_US
dc.rights	CC Attribution-Noncommercial-No Derivative Works 2.5 India	en_US
dc.source	BVAAP Vol.19(1) [June 2011]	en_US
dc.title	Protoplast formation and regeneration of Acetobacter pasteurianum NCIM 2314	en_US
dc.type	Article	en_US
Appears in Collections:	BVAAP Vol.19(1) [June 2011]

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