Pectin lyases of a few indigenous fungal strains

Abstract

Four pectin lyases (PNLs), producing indigenous fungal strains (Aspergillus flavus, A. niger, A. phoenicis and A. wentii), have been isolated from local samples. Enzymatic characteristics of PNLs produced by above fungal strains using citrus pectin have been found to be (respectively): Km, 0.38, 0.67, 0.55 and 0.32 mg/ml; pH, 8.0, 7.0, 5.0 and 7.0; and temperature, 50 °C for all PNLs. Effect of metal ions (Ag⁺, Ca⁺⁺, Co⁺⁺, Cu⁺⁺, Hg⁺⁺, K⁺, Mg⁺⁺, Zn⁺⁺, Na⁺) and protein inhibitors (EDTA, sodium arsenate, sodium azide, potassium permanganate and potassium ferrocyanide) on the activities of PNLs has been determined.