Ligninolytic enzyme production under solid-state fermentation by white rot fungi

Abstract

Ligninolytic enzymes production has been studied using various white rot fungi, Phanerocheate chrysosporium, Pleurotus ostreatus, Tramates versicolor, Irpex lacteus under solid-state fermentation. All the fungi produced and secreted oxidative enzymes were associated with lignin degradation. Among various substrates (wheat straw, corncobs, coconut coir, wheat bran, and rice bran), wheat straw was found to be the best for laccase, lignin peroxidase and manganese peroxidase production. Maximum enzyme activity of laccase by P. ostreatus was obtained on 8th day of fermentation. Manganese peroxidase and lignin peroxidase showed highest activity on 10th day of fermentation. The level of enzyme activity was compared with submerged fermentation for P.ostreatus with wheat straw as the substrate.