Study on 16s rRNA based PCR method for specific detection of <i>Salmonella entrica typhi</i> from gut of infected silkworm <i>Bombyx mori (Linn.)</i>

Pradhap, M; Selvisabhanayakam; Mathivanan, V; Parthasarathy, V; Ayyappan, J V Ananda; Kumar, S Sathees

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Please use this identifier to cite or link to this item: http://nopr.niscpr.res.in/handle/123456789/12959

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DC Field	Value	Language
dc.contributor.author	Pradhap, M	-
dc.contributor.author	Selvisabhanayakam	-
dc.contributor.author	Mathivanan, V	-
dc.contributor.author	Parthasarathy, V	-
dc.contributor.author	Ayyappan, J V Ananda	-
dc.contributor.author	Kumar, S Sathees	-
dc.date.accessioned	2011-10-31T10:08:17Z	-
dc.date.available	2011-10-31T10:08:17Z	-
dc.date.issued	2011-11	-
dc.identifier.issn	0975-1084 (Online); 0022-4456 (Print)	-
dc.identifier.uri	http://hdl.handle.net/123456789/12959	-
dc.description	909-911	en_US
dc.description.abstract	This study presents a novel molecular method for detection of Salmonella entrica typhi strain based on 16s rRNA sequence analysis by modification of PCR primer 16sF1, which was combined with a newly developed primer. Annealing method at the position of 50°C PCR amplification of 16s rRNA was performed with universal primer 27F and 1429R. Combination of these two specific primers proved specific detection of Salmonella entrica typhi, which was isolated and cultured from gut of silk worm Bombyx mori (Linn.).	en_US
dc.language.iso	en_US	en_US
dc.publisher	NISCAIR-CSIR, India	en_US
dc.rights	CC Attribution-Noncommercial-No Derivative Works 2.5 India	en_US
dc.source	JSIR Vol.70(11) [November 2011]	en_US
dc.subject	PCR	en_US
dc.subject	Primer	en_US
dc.subject	Primer annealing	en_US
dc.subject	Proteobacteria	en_US
dc.subject	Salmonella	en_US
dc.title	Study on 16s rRNA based PCR method for specific detection of Salmonella entrica typhi from gut of infected silkworm Bombyx mori (Linn.)	en_US
dc.type	Article	en_US
Appears in Collections:	JSIR Vol.70(11) [November 2011]

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