Please use this identifier to cite or link to this item: http://nopr.niscpr.res.in/handle/123456789/12964
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dc.contributor.authorPonmani, Thangaraj-
dc.contributor.authorMunavar, M Hussain-
dc.date.accessioned2011-10-31T10:14:05Z-
dc.date.available2011-10-31T10:14:05Z-
dc.date.issued2011-11-
dc.identifier.issn0975-1084 (Online); 0022-4456 (Print)-
dc.identifier.urihttp://hdl.handle.net/123456789/12964-
dc.description938-944en_US
dc.description.abstractThis study presents in vivo role of Cra (Catabolite repressor activator) protein in regulation of two selected genes (prpB & yahA) of Escherichia coli. Gene prpB bears Cra binding site and yahA region binds to Cra in vitro. Therefore, in pBR322 background, pprpB-lacZ and pyahA-lacZ transcriptional fusions were generated and studied expression of b-galactosidase from both fusions in cra+ and cra¯ genetic backgrounds of E. coli. Results that both the promoters could possibly be up-regulated by Cra in vivo in E. coli were discussed in relation to transcription control by accessory transcription factor(s).en_US
dc.language.isoen_USen_US
dc.publisherNISCAIR-CSIR, Indiaen_US
dc.rights CC Attribution-Noncommercial-No Derivative Works 2.5 Indiaen_US
dc.sourceJSIR Vol.70(11) [November 2011]en_US
dc.subjectCraen_US
dc.subjectlacZen_US
dc.subjectprpBen_US
dc.subjectyahAen_US
dc.subjectTranscriptional fusionen_US
dc.titleAssessing role of Cra in regulation of prpB and yahA genes of Escherichia coli in vivo using lacZ transcriptional fusionsen_US
dc.typeArticleen_US
Appears in Collections:JSIR Vol.70(11) [November 2011]

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