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Title: Impact of infectious Candida albicans biofilm on biomaterials
Authors: Singh, Nivedita
Agrawal, Vishnu
Pemmaraju, Suma C
Panwar, Richa
Pruthi, Vikas
Keywords: Biofilm;Biomaterials;Candida albicans;CLSM;XTT
Issue Date: Oct-2011
Publisher: NISCAIR-CSIR, India
Abstract: In the present investigation, biofilm formation by Candida albicans was studied on different polymeric surfaces, viz., polypropylene (PP), polystyrene (PS), polyvinylchloride (PVC), and silicone rubber (SR). Amongst these polymeric surfaces, the maximum biofilm formation was recorded to be 64.19, 50.31, and 45.09% for PS, PP, SR, respectively in comparison to PVC after 48 h using XTT [2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)2H-tetrazolium-carboxanilide] tetrazolium reduction assay. Exopolysaccharides (EPS) production during biofilm formation, when assessed using acetone precipitation technique, was found to be 11.45, 9.41, 8.65 and 6.95 g/cm2 for PVC, PS, PP and SR, respectively. Atomic force microscopic and goniometric analysis showed maximum roughness (134 nm) and hydrophobicity (97°) for PVC. Confocal laser scanning microscopy (CLSM) studies revealed maximum biofilm thickness (117.5 µm) on PVC surface when analyzed by z-sectioning. Further, the data were confirmed by scanning electron microscopy (SEM) for biofilm growth on these biomaterials. It was observed that PVC as biomaterial is most susceptible for C. albicans biofilm formation, while material surface properties like roughness and hydrophobicity promotes C. albicans adhesion and biofilm development.
Page(s): 417-422
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.10(4) [October 2011]

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