<span style="font-size:11.0pt;font-family: "Times New Roman","serif";mso-fareast-font-family:"Times New Roman";mso-bidi-font-family: Mangal;mso-ansi-language:EN-GB;mso-fareast-language:EN-US;mso-bidi-language: HI" lang="EN-GB">Staurosporine induced poly (ADP-ribose) polymerase independent cell death in <i style="mso-bidi-font-style:normal">Dictyostelium discoideum</i></span>

Abstract

In the present study <i style="mso-bidi-font-style: normal">D. discoideum </i>has been used as a model organism to understand the role of poly (ADP-ribose) polymerase (PARP) in caspase independent paraptotic cell death pathways. <i style="mso-bidi-font-style:normal">D. discoideum</i> lacks caspases and Bcl-2 family proteins; nevertheless it has 9 potential genes for PARP. PARP has been known to get activated in various cell death associated diseases. In this study kinetics of cell death induced by staurosporine (STS), a bacterial alkaloid, was established to unravel the role of PARP. It was found that STS induced cell death in <i style="mso-bidi-font-style:normal">D. discoideum</i> did not involve PARP activation, however it involved cathepsin D. Results indicated that an alternative mechanism may be existing in <i style="mso-bidi-font-style:normal">D. discoideum </i>that<i style="mso-bidi-font-style: normal"> </i>lacks Bcl-2 family proteins for STS induced cell death that evades Bax involvement.