An improved method of DNA isolation from polysaccharide rich leaves of <i>Boswellia serrata </i>Roxb.

Abstract

A quick, simple and improved DNA extraction method from polysaccharide rich leaves of <i>Boswellia serrata</i> Roxb. has been developed. Different methods involving use of CTAB and SDS with or without modification were used. A CTAB based extraction method which uses diatomite to remove polysaccharides, such as, gums and resin, proved to be the best. This method allowed recovery of good quality DNA in sufficient quantity, suitable for complete digestion by restriction endonucleases and amplifiable in polymerase chain reaction as compared to other methods.