<i style="">In vitro</i> plant regeneration from immature seeds of <i style="">Murraya koenigii</i> (L.) Spreng.

Abstract

An efficient and reproducible <i style="">in vitro</i> protocol was developed for the large scale propagation of <i style="">Murraya koenigii</i> (L.) Spreng. from immature seeds. Direct shoot proliferation was achieved from intact seedlings on Murashige and Skoog (MS) medium supplemented with BAP (2.5 mg L^-1). The raised multiple shoots (19.85) were dwarf. Therefore, they were transferred to shoot elongation medium comprising of MS salts fortified with GA₃ (0.4 mg L^-1). The elongated shoots (5.6 cm) were then rooted on ½ MS medium supplemented with IAA (0.5 mg L^-1). The plantlets were hardened and acclimatized in plant growth chamber at 26±2°C temperature and 80% humidity under 16/8 h light and dark photoperiod by cool white fluorescent tube light of about 3000 lux. Finally, the plantlets were transferred to field conditions, where they survived with 80% survival rate.