Optimization of extraction conditions of total phenol from lotus (<i>Nelumbo nuficera </i>Gaertn) rhizome

Abstract

This study presents operating factors affecting yield of total phenol and ferric reducing antioxidant power (FRAP) value of extracts from lotus rhizome using orthogonal design experiment (L₉(3⁴)) based on single factor experiments. Optimum extraction conditions on yield of total phenol and FRAP value, respectively, were found as follows: ratio of material to liquid, 1 g: 6 ml, 1 g: 8 ml; extraction temperature, 70^oC, 50^oC; extraction time, 2 h, 0.5 h; and ethanol concentration, 40%, 40%.