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http://nopr.niscpr.res.in/handle/123456789/14011| Title: | Analysis of chitinase gene specific transcript accumulation in tea [Camellia sinensis (L.) O. Kuntze] during induced systemic resistance by methyl jasmonate |
| Authors: | Roy, S C Chakraborty, B N |
| Keywords: | Camellia sinensis;chitinase gene transcript;ISR;RT-PCR |
| Issue Date: | Apr-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Chitinase gene (Pr-3) specific transcript accumulation was increased by 5.57% in mature leaf compared to the other in vitro grown tissues in tea (Camellia sinensis) genotype (T383) during induced systemic resistance by methyl jasmonate. Chitinase (Pr-protein) gene specific two primers were used in RT-PCR reaction to measure the differential transcript accumulation in different tissue systems (young leaf, mature leaf, callus and shoot regenerated from somatic embryo). The time-course expression patterns resulted in differential expression of two transcripts (254 & 366 bp) in response to induction. The 366 bp transcript was sequenced bidirectionally and deposited into the GenBank of NCBI (acc.no. EU373553). |
| Page(s): | 142-147 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Appears in Collections: | IJBT Vol.11(2) [April 2012] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJBT 11(2) 142-147.pdf | 314.6 kB | Adobe PDF | View/Open |
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