Detection of genetically modified cotton seeds using PCR and real-time PCR

Abstract

Detection of genetically modified (GM) crops and products are necessary to comply with international labeling regulations and to avoid spurious and unapproved GM planting. DNA based analytical tools involving PCR and real-time PCR were used to detect GM cotton seeds. Four pairs of primers specific for <i style="">CaMV </i>35S, <i style="">Nos, nptII </i>and <i style="">cry1Ac </i>genes were used for the DNA detection of GM crop. The limit of detection in real-time PCR was found to be 0.1%. Evidently, these two PCR techniques were successful in detecting transgenes in the DNA of GM cotton seeds.