High frequency <i>in vitro</i> shoot regeneration of <i>Clitoria ternatea</i> L. affected by different cultural conditions

Abstract

An efficient <i>in vitro</i> propagation system was established for direct shoot regeneration in <i>Clitoria ternatea</i> L.. The regeneration protocol was standardized by using different explants, viz., nodal, cotyledonary node and shoot tip, on Murashige and Skoog (MS) medium with different concentrations of sucrose (1-6% ), plant growth regulators BA and Kn (1.0-6.0 µ<i>M</i>), and different levels of <i>p</i>H (5.2-6.2). The highest mean number of shoots (18.7±1.0) was obtained from nodal explants on MS medium containing 3% sucrose and 0.8% agar supplemented with BA (5.0 µ<i>M</i>) at <i>p</i>H 5.8. <i>In vitro</i> regenerated and elongated shoots were rooted on ½ MS medium fortified with IBA (2.0 µ<i>M</i>). Complete plantlets were then hardened, acclimatized and transplanted to natural conditions, where they exhibited 80% survivability.