<i style="">In vitro</i> antibacterial activity of leaf extracts of <i style="">Rhizophora mucronata </i>L. against multi drug resistant <i style="">Vibrio</i> spp. isolated from marine water Lobster’s larvae hatcheries

Abstract

Present article consists the <i style="">in vitro</i> antibacterial study carried out using leaf extracts of <i style="">Rhizophora mucronata</i> L., a mangrove plant. Extracts were screened for antibacterial activity against multi-drug resistant <i style="">Vibrio</i> <i style="">harveyi</i> and <i style="">Vibrio campbellii</i>. The same were isolated from Lobster’s larvae hatchery water. Antibiotic resistance and sensitivity of the isolated <i style="">Vibrio</i> spp. were tested with Hi-Media antibiotic disc. Out of total eight antibiotics discs used, <i style="">V.</i> <i style="">harveyi</i>

was found highly resistant to ampicillin, oxacillin, cephalothin, vancomycin, erythromycin, and clidamycin, while, it was sensitive towards chloramphenicol (19 mm) and gentamycin (9 mm). Whereas, <i style="">Vibrio campbellii</i> was found highly resistant to ampicillin, oxacillin, cephalothin, vancomycin, and erythromycin. <i style="">Vibrio campbellii</i> was highly sensitive to chloramphenicol (20 mm), and moderately sensitive to clindamycin (9 mm), and gentamycin (14 mm). Chloroform leaf extract of <i style="">Rhizophora mucronata</i> L. was found to be the most active one against both <i style="">Vibrio </i>spp., while, hexane, ethyl acetate, and methanol extract revealed moderate activity against both <i style="">Vibrio</i> spp. However, distilled water extract did not have any activity. Chloromphenicol from the Hi-Media antibiotic and chloroform extract from <i style="">Rhizophora mucronota</i> leaves can be used as a source of alternative compound to control the <i style="">Vibrio</i> spp. which infects the Lobster’s larvae.