<span style="font-size:15.0pt;font-family: "Times New Roman";mso-fareast-font-family:"Times New Roman";mso-ansi-language: EN-US;mso-fareast-language:EN-US;mso-bidi-language:AR-SA;mso-bidi-font-weight: bold;mso-bidi-font-style:italic" lang="EN-US">Evaluation of antioxidant properties<span style="font-size:15.0pt;font-family:"Times New Roman";mso-fareast-font-family: "Times New Roman";mso-ansi-language:EN-US;mso-fareast-language:EN-US; mso-bidi-language:AR-SA;mso-bidi-font-weight:bold" lang="EN-US"> of <i>Tabernaemontana heyneana </i>Wall. leaves</span></span>

Abstract

<span style="font-size:11.0pt;mso-bidi-font-size: 10.0pt;font-family:" times="" new="" roman";mso-fareast-font-family:"times="" roman";="" mso-ansi-language:en-us;mso-fareast-language:en-us;mso-bidi-language:ar-sa"="" lang="EN-US">The leaf extract of <i>Tabernaemontana heyneana </i>Wall. from different solvents was investigated for <i>in vitro</i> antioxidant activities. The antioxidant stability of the extracts under preserved condition (one month stored at 4ºC) was also analyzed. The fresh extracts were found to contain appreciable total polyphenolic content (11.4 ± 0.17 mg/g tissue), total flavonoids (4.3 ± 0.17 mg rutin equivalents/g tissue) and ascorbic acid (5.0 ± 0.01 mg/ g tissue). The methanolic leaf extract of <i>T. heyneana </i>Wall. (METH-L) possesses remarkable reducing power [ferrous, cupric and cerium (IV) ions]. The metal ions were chelated at IC₅₀ = 549 μg/ml and hydroxyl, ABTS and DPPH radicals were inhibited at IC₅₀= 577 μg/ml, 537 μg/ml and 507 μg/ml, respectively. A noteworthy superoxide (<span style="font-size:11.0pt;mso-bidi-font-size:10.0pt;font-family:" times="" new="" roman";="" mso-fareast-font-family:"times="" roman";color:black;mso-ansi-language:en-us;="" mso-fareast-language:en-us;mso-bidi-language:ta"="" lang="EN-US">69.07% ± 11.88<span style="font-size:11.0pt;mso-bidi-font-size:10.0pt;font-family:" times="" new="" roman";="" mso-fareast-font-family:"times="" roman";mso-ansi-language:en-us;mso-fareast-language:="" en-us;mso-bidi-language:ar-sa"="" lang="EN-US">) and nitric oxide (<span style="font-size:11.0pt;mso-bidi-font-size:10.0pt;font-family:" times="" new="" roman";="" mso-fareast-font-family:"times="" roman";color:black;mso-ansi-language:en-us;="" mso-fareast-language:en-us;mso-bidi-language:ta"="" lang="EN-US">74.73 % ± 10.12<span style="font-size:11.0pt;mso-bidi-font-size:10.0pt;font-family:" times="" new="" roman";="" mso-fareast-font-family:"times="" roman";mso-ansi-language:en-us;mso-fareast-language:="" en-us;mso-bidi-language:ar-sa"="" lang="EN-US">) radical scavenging activities were observed in METH-L.<span style="font-size:11.0pt;mso-bidi-font-size:10.0pt; font-family:" times="" new="" roman";mso-fareast-font-family:"times="" roman";="" color:black;mso-ansi-language:en-us;mso-fareast-language:en-us;mso-bidi-language:="" ta"="" lang="EN-US"> <span style="font-size:11.0pt;mso-bidi-font-size:10.0pt; font-family:" times="" new="" roman";mso-fareast-font-family:"times="" roman";="" mso-ansi-language:en-us;mso-fareast-language:en-us;mso-bidi-language:ar-sa"="" lang="EN-US">Similarly, the preserved leaf extracts have proved to possess a stable antioxidant activity compared to fresh extracts (FRAP=1.25% decrease, CUPRAC = 3.88% decrease, metal chelation =3.36% decrease and DPPH = 2.05% decrease). The overall scientific documentation indicated that the METH-L<span style="mso-bidi-font-style:italic"> possess significant antioxidant activity than the other solvent extracts. </span></span></span></span></span></span></span></span>