A new method for determination of ascorbic acid in fruit juices, pharmaceuticals and biological samples

Abstract

This study presents a new, selective and accurate indirect spectrophotometric method for determination of L-ascorbic acid in fruit juices, pharmaceuticals and biological samples. Beer’s law is obeyed by ascorbic acid (conc. range, 0.8-6.3 <span style="font-family:Symbol;mso-bidi-font-family:Symbol">mg) per 25 ml of final solution (0.032-0.252 ppm). The method showed: apparent molar absorptivity, 1.56 × 10⁵l mol^-1cm^-1; Sandell’s sensitivity, 0.0024 <span style="font-family:Symbol;mso-bidi-font-family: Symbol">mg cm^-2; limit of detection, 0.0086 <span style="font-family:Symbol;mso-bidi-font-family:Symbol">mg ml^-1; and limit of quantitation, 0.01 <span style="font-family:Symbol;mso-bidi-font-family: Symbol">mg ml^-1. It was satisfactorily applied for determination of ascorbic acid in fruit juices, pharmaceuticals and biological samples. Reliability of method was established by parallel determination against Leuco crystal violet and Rhodamine – B method.

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