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http://nopr.niscpr.res.in/handle/123456789/14531| Title: | A superoxide anion electrochemical sensor based on the direct electrochemistry of superoxide dismutase assembled layer-by-layer at the L-cysteine modified gold electrode |
| Authors: | Yang, Yan Zhou, Jiawan Wu, Liang Zhang, Xiaohua Chen, Jinhua |
| Keywords: | Superoxide anion;Superoxide dismutase;Electrochemistry;Poly-(diallyldimethylammonium);Sensors;Modified electrodes;Layer-by-layer assembly |
| Issue Date: | Aug-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | A superoxide anion (O2•–) biosensor has been developed based on layer-by-layer assembled poly-(diallyldimethylammonium) (PDDA) and superoxide dismutase (SOD) on the L-cysteine (Cys) modified gold (Au) electrode. In 25 mM phosphate buffer solution (pH 7.2), the {SOD/PDDA}n/Cys/Au electrode shows a pair of well-defined and nearly reversible peak at ~ 84 mV vs SCE, confirming the direct electrochemistry of SOD. Based on the bifunctional enzymatic catalytic activities of SOD to the oxidation and reduction of O2•–, the developed {SOD/PDDA}5/Cys/Au electrode exhibits good analytical characteristics in the detection of O2•–, such as low detection limit, good stability, reproducibility, selectivity and particularly a wide linear range (0.5 ~ 546 mM). |
| Page(s): | 1057-1063 |
| ISSN: | 0975-0975(Online); 0376-4710(Print) |
| Appears in Collections: | IJC-A Vol.51A(08) [August 2012] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJCA 51A(8) 1057-1063.pdf | 329.41 kB | Adobe PDF | View/Open |
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