<span style="font-size:11.0pt;font-family: "Times New Roman";mso-fareast-font-family:"Times New Roman";mso-bidi-font-family: Mangal;mso-ansi-language:EN-GB;mso-fareast-language:EN-IN;mso-bidi-language: HI" lang="EN-GB">ARMS-PCR as an alternative, cost effective method for detection of <i style="mso-bidi-font-style:normal">FecB</i> genotype in sheep</span>

Abstract

<span style="mso-fareast-language:EN-IN; mso-bidi-font-weight:bold" lang="EN-GB">Authors<span style="mso-ansi-language: EN-IN;mso-fareast-language:EN-IN" lang="EN-GB"> <span style="mso-ansi-language:EN-IN; mso-fareast-language:EN-IN">have standardized and report here a simplified amplification refractory mutation system<span style="mso-ansi-language:EN-IN;mso-fareast-language:EN-IN"> (ARMS)-<span style="mso-ansi-language:EN-IN;mso-fareast-language: EN-IN">PCR test as an alternative technique to PCR-RFLP for detection of <i style="mso-bidi-font-style:normal">FecB</i> genotype in sheep. <span style="mso-ansi-language:EN-IN;mso-fareast-language:EN-IN">An ARMS<span style="mso-ansi-language:EN-IN;mso-fareast-language: EN-IN">-PCR test to detect the <i style="mso-bidi-font-style:normal">FecB</i> mutation in Black Bengal goats has recently been described in the literature. A number of modifications to this technique has been made to simplify and standardize the protocol to use it for sheep DNA, isolated from blood samples on FTA paper. The modifications included reducing the <span style="mso-bidi-font-weight:bold" lang="EN-GB">PCR reaction volume to half of original protocol, using a single touchdown annealing temperature and combining two PCR amplicons amplified with different primer pairs (one for each allele of <i style="mso-bidi-font-style:normal">FecB</i>) for each sample prior to loading on the gel. These modifications can make the technique cheaper and more suitable for large scale, rapid <i style="mso-bidi-font-style: normal">FecB</i> genotyping in sheep for use in <i style="mso-bidi-font-style: normal">FecB</i> introgression breeding programmes.

</span></span></span></span></span></span></span></span>