<span style="font-size:11.0pt;font-family: "Times New Roman";mso-fareast-font-family:"Times New Roman";mso-bidi-font-family: Mangal;mso-ansi-language:EN-GB;mso-fareast-language:EN-US;mso-bidi-language: HI" lang="EN-GB">Rapid <i style="mso-bidi-font-style:normal">in vitro</i> mass multiplication of orchids <i style="mso-bidi-font-style:normal">Aerides maculosa</i> <span style="font-size:11.0pt;font-family:"Times New Roman"; mso-fareast-font-family:Calibri;mso-bidi-font-family:Mangal;mso-ansi-language: EN-GB;mso-fareast-language:EN-US;mso-bidi-language:HI" lang="EN-GB">Lindl. <span style="font-size:11.0pt;font-family:"Times New Roman";mso-fareast-font-family: "Times New Roman";mso-bidi-font-family:Mangal;mso-ansi-language:EN-GB; mso-fareast-language:EN-US;mso-bidi-language:HI" lang="EN-GB">and <i style="mso-bidi-font-style: normal">Rhynchostylis retusa</i> (L.) <span style="font-size: 11.0pt;font-family:"Times New Roman";mso-fareast-font-family:Calibri; mso-bidi-font-family:Mangal;mso-ansi-language:EN-GB;mso-fareast-language:EN-US; mso-bidi-language:HI" lang="EN-GB">Bl. from immature seeds</span></span></span></span>

Abstract

The callus embryoid plant regeneration was developed using immature seeds of <i>Aerides maculosa</i> Lindl. and <i>Rhynchostylis retusa</i> (L.) Bl. Immature capsules of 5-10 wk after pollination (<i style="mso-bidi-font-style:normal">A. maculosa</i>) and<i style="mso-bidi-font-style: normal"> </i>10-20 wk after pollination (<i style="mso-bidi-font-style:normal">R. retusa</i>)<i style="mso-bidi-font-style:normal"> </i>were used for callus induction. In both the species, best response of callus induction was observed on Vacin and Went (VW) medium supplemented with 15% coconut water (CW, v/v). In <i style="mso-bidi-font-style:normal">A. maculosa</i>,<i style="mso-bidi-font-style: normal"> </i>maximum number of protocorm-like bodies (PLBs; 13.93 per callus) was noted on VW medium supplemented with BA (1 mg L^-1)+NAA (1 mg L^-1) + 15% CW. While in <i style="mso-bidi-font-style:normal">R. retusa</i>, maximum number of PLBs (15.83 per callus) was noted in Murashige and skoog (MS) medium supplemented with BA (1 mg L^-1)+NAA (1 mg L^-1)+15% CW. <i style="mso-bidi-font-style:normal">In vitro</i> regenerated plantlets of both the species were acclimatized to green house conditions. The highest survival rate of plantlets (60%) was noted in the combination of charcoal pieces, brick pieces (1:1) and coconut husk.