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http://nopr.niscpr.res.in/handle/123456789/14580| Title: | In vitro shoot multiplication from nodal explants of Boucerosia diffusa Wight—An endemic medicinal plant |
| Authors: | Ramadevi, T Ugraiah, A Pullaiah, T |
| Keywords: | Apocynaceae;Boucerasia diffusa;In vitro propagation;Plant growth regulators |
| Issue Date: | Jul-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | In vitro propagation of Boucerosia diffusa Wight (Apocynaceae), an endemic medicinal plant of Western Ghats, (Tamil Nadu, India) was carried out. Nodal explants were cultured on MS medium supplemented with cytokinins [benzyl aminopurine (BAP), kinetin (Kn), thidiazuron (TDZ) & zeatin] either individually or in combinations. Shoot buds proliferated on MS medium+BAP (8.87 µM) and repeated subculture on MS+BAP (8.87 µM)+TDZ (2.27 µM) promoted the shoot multiplication. The regenerated shoots were then rooted on MS+NAA (2.69 µM). The micropropagated planlets were hardened with the survival success rate of 56%. The developed micropropagation protocol would help ex situ conservation of this endemic species. |
| Page(s): | 344-347 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Appears in Collections: | IJBT Vol.11(3) [July 2012] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJBT 11(3) 344-347.pdf | 119.66 kB | Adobe PDF | View/Open |
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