Spectrofluorimetric study on inclusion interaction of β-cyclodextrin with duloxetine and its analytical application

Abstract

<span style="mso-ansi-language:EN-US" lang="EN-US">Fluorescence study on inclusion interaction of duloxetine in β-cyclodextrin shows significant increase in the fluorescence of duloxetine in the presence of β-cyclodextrin. The effects of <i style="mso-bidi-font-style: normal">p</i>H and cyclodextrin concentration on the fluorescence spectra are reported. Fluorescence spectroscopy of the host-guest interaction between duloxetine and β-cyclodextrin shows formation of inclusion complex with a 1:1 stoichiometric ratio. The changes in fluorescence of duloxetine on inclusion in the hydrophobic β-cyclodextrin cavity is used to calculate its association constants by non-linear regression method. A new spectrofluorometric method is proposed for quantitative determination of duloxetine in dosage forms with limit of detection of 2.0 × 10^-8mol/L. The linear range is 5.16 × 10^-8 mol/L to 1 × 10^-5 mol/L and correlation coefficient is 0.9998. The accuracy (recovery 99.5–100.8 %) and precision (RSD 0.58–0.89 %) values of the proposed method are satisfactory.

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