Purification and properties of invertase from the flowers of <i>Woodfordia fruticosa</i>

Weersaooriya, M K B; Yatawara, H P

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Please use this identifier to cite or link to this item: http://nopr.niscpr.res.in/handle/123456789/15212

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DC Field	Value	Language
dc.contributor.author	Weersaooriya, M K B	-
dc.contributor.author	Yatawara, H P	-
dc.date.accessioned	2012-12-08T16:15:45Z	-
dc.date.available	2012-12-08T16:15:45Z	-
dc.date.issued	2002-10	-
dc.identifier.issn	0975-0959 (Online); 0301-1208 (Print)	-
dc.identifier.uri	http://hdl.handle.net/123456789/15212	-
dc.description	347-350	en_US
dc.description.abstract	Invertase (β- fructofuranosidase, EC 3.2.1.26) was purified from the flowers of of Woodfordia fruticosa, which is used to prepare certain fermented Ayurvedic drugs. The enzyme was purified to near homogeneity as judged by native PAGE with a yield of 10.7%, using (N H₄)₂SO₄ fractionation, followed by gelfiltration through Sepharose 4B and DEAE cellulose chromatography at pH 6.8 and 4.42. The molecular mass of the purified enzyme as determined by elution through Sepharose 4B gel column was found to be ~ 280 kDa. SDS-PAGE of the purified enzyme showed that the enzyme is composed of three subunits with molecular mass of 66. 43 and 40 kDa. The enzyme showed a broad pH optimum between 4.0-7.0. Optimum assay temperature was 37°C and above 45°C, the enzyme activity slowly declined and inactivated around 80°C. The apparent K_m value of th e enzyme for sucrose was 160mM.	en_US
dc.language.iso	en_US	en_US
dc.publisher	CSIR	en_US
dc.rights	CC Attribution-Noncommercial-No Derivative Works 2.5 India	en_US
dc.source	IJBB Vol.39(5) [October 2002]	en_US
dc.title	Purification and properties of invertase from the flowers of Woodfordia fruticosa	en_US
dc.type	Article	en_US
Appears in Collections:	IJBB Vol.39(5) [October 2002]

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