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http://nopr.niscpr.res.in/handle/123456789/15272Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Dubey, Ashok K | - |
| dc.date.accessioned | 2012-12-22T20:00:21Z | - |
| dc.date.available | 2012-12-22T20:00:21Z | - |
| dc.date.issued | 2002-08 | - |
| dc.identifier.issn | 0975-0959 (Online); 0301-1208 (Print) | - |
| dc.identifier.uri | http://hdl.handle.net/123456789/15272 | - |
| dc.description | 279-283 | en_US |
| dc.description.abstract | Preparations of recombinant envelope glycoprotein E2 of hepatitis C virus (r-HCV E2), found to be homogeneous by N-terminal amino acid sequencing and mass spectrometry, resolved into multiple ionic species (isoforms) when analysed by isoelectric focusing (IEF) gel electrophoresis in the pI range of 3-10. These isoforms possessed pI values in the range or 4.5-8.2. The major isoform with pI value of approximately 7. 1 was separated from the rest of them by employing a method developed on Gradiflow BF 200, a device based on preparative electrophoresis. This isoform was adjudged to be homogenous by IEF and by native polyacrylamide gel electrophoresis (PAGE). | en_US |
| dc.language.iso | en_US | en_US |
| dc.publisher | NISCAIR-CSIR, India | en_US |
| dc.rights |  CC Attribution-Noncommercial-No Derivative Works 2.5 India | en_US |
| dc.source | IJBB Vol.39(4) [August 2002] | en_US |
| dc.title | Resolution of a complex ionic mixture of an apparently homogenous protein preparation by preparative electrophoresis | en_US |
| dc.type | Article | en_US |
| Appears in Collections: | IJBB Vol.39(4) [August 2002] | |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJBB 39(4) 279-283.pdf | 1 MB | Adobe PDF | View/Open |
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