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http://nopr.niscpr.res.in/handle/123456789/15289| Title: | Rat brain membrane-bound delta opioid receptor: Loss and reactivation of binding on dialysis and aging at low temperature |
| Authors: | Christadoss, E Oommen, A |
| Issue Date: | Jun-2001 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | A change in the environment of rat brain membranes by dialysis from phosphate buffered saline (PBS) to 10 mM potassium phosphate (pH 7.2) led to a 35% loss in delta opioid receptor binding. while alteration of membrane structure on freezing at -20°C for 55 days led to 85% loss of receptor binding. The dialysate. 200 mM KCI and NaCI restored receptor binding lost on dialysis. This K+ and Na+ restabilisation of the receptor can be through cation-π bonding. interactions that are Suited the lipid bilayer. In membranes stored at -20°C, the loss or binding is attributed to increased membrane fluidity by phospholipase A2 action on membrane phospholipids. resulting in an increase of free fatty acids. K+ but not Na+ restabilization of these membrane receptors may be due to the ability or K+ 10 decrease membrane fluidity. |
| Page(s): | 166-169 |
| ISSN: | 0975-0959 (Online); 0301-1208 (Print) |
| Appears in Collections: | IJBB Vol.38(3) [June 2001] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJBB 38(3) 166-169.pdf | 982.96 kB | Adobe PDF | View/Open |
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