Please use this identifier to cite or link to this item:
http://nopr.niscpr.res.in/handle/123456789/15293| Title: | Purification and characterization of a hemagglutinin isolated from the leaves of Chenopodium (Chenopodium amaranticolor) |
| Authors: | Suseelan, K N Mitra, R |
| Issue Date: | Jun-2001 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | A hemagglutinin was isolated and purified from the leaves of Chenopodium (Chenopodium amaranticolor) using ionexchange chromatography and affinity chromatography on fetuin- agarose matrix . It agglutinated rabbit erythrocytes. The hemagglutinin had a native molecular mass of 58 kDa, as estimated by gel filtration and showed a single band of molecular mass of 33 kDa on SDS-PAGE. It showed hemagglutinati on activity over the pH range 3-12 and was found to be stable up to 70°C. On isoelectrofocussing, the pl of this hemagglutinin was estimated to be 5.25. However, it was found to contain seven charge variants when isoelectrofocussing was performed in presence of 6M urea. |
| Page(s): | 186-192 |
| ISSN: | 0975-0959 (Online); 0301-1208 (Print) |
| Appears in Collections: | IJBB Vol.38(3) [June 2001] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJBB 38(3) 186-192.pdf | 1.35 MB | Adobe PDF | View/Open |
Items in NOPR are protected by copyright, with all rights reserved, unless otherwise indicated.