Multiple isoforms of protein kinase C in lymphocytes and airway smooth muscle of guinea pig

Abstract

The isoenzyme pattern of protein kinase C (PKC) in lymphocytes and airway smooth muscles (ASM) was examined by Wes tern blot using commercially available monoclonal

antibodies. The results showed the presence of PKC α, β, γ, ε, η, μ and ζ in lymphocytes and PKC α, γ, ε, η and ζ in ASM. The unexpected feature was the presence of PKC γ in both lymphocytes and ASM of guinea pigs. Expression of this PKC isoform is usually restricted to tissues in the central nervous system or spinal cord. Expression of PKC δ, θ, λ and τ was not detected in either lymphocytes or ASM.