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http://nopr.niscpr.res.in/handle/123456789/15327| Title: | Effect of γ-irradiation on H3 histone and DNA in solution |
| Authors: | Upadhyay, S N |
| Issue Date: | Dec-2001 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Three methods, namely, absorbance of colour by reaction with Folin-Ciocalteau reagent, UV absorbance and fluorescence intensity measurements for detection of H3 histone in 0.15 M standard saline citrate (SSC) solution were compared. Maximum sensitivity was found with the Folin-Ciocalteau method. Effect of varying pH and of γ - radiation on H3 hi stone and on interaction of H3 hi stone with DNA were studied. For this, solutions of H3 histone in SSe. in 0.9% NaCl, H3 hi stone + DNA in 0.9% NaCI were subjected to varying pH (1-10) and γ - radiation (dose 10-50 Gy) and λmax and Aλmax),max were monitored. From the molar ratios of histone and DNA in the complex, it was observed that at γ-radiation dose of 50 Gy and pH 8.54, there was a depletion of 6-8 μg/ml of histone from the histone-DNA complex . |
| Page(s): | 406-411 |
| ISSN: | 0975-0959 (Online); 0301-1208 (Print) |
| Appears in Collections: | IJBB Vol.38(6) [December 2001] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJBB 38(6) 406-411.pdf | 1.15 MB | Adobe PDF | View/Open |
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