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http://nopr.niscpr.res.in/handle/123456789/15373| Title: | Urate oxidase electrode based on dissolved oxygen probe for urine uric acid, Determination |
| Authors: | Dinckaya, Erhan Akyilmaz, Erol Akgol, Sinan |
| Issue Date: | Feb-2000 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | A biosensor for the specific determination of uric acid in urine was developed using urate oxidase (EC 1.7.3.3) in combination with a dissolved oxygen probe. Urate oxidase was immobilized with gelatin by means of glutaraldehyde and fixed on a pretreated teflon membrane to serve as enzyme electrode. The electrode response was maximum when 50 mM glycine buffer was used at pH 9.2 and 35°C. The enzyme electrode response depends linearly on uric acid concentration between 5- 40 μM with a response time of 5 min. The enzyme electrode is stable for more than 2 weeks and during this period over 35 assays were performed. |
| Page(s): | 67-70 |
| ISSN: | 0975-0959 (Online); 0301-1208 (Print) |
| Appears in Collections: | IJBB Vol.37(1) [February 2000] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJBB 37(1) 67-70.pdf | 758.42 kB | Adobe PDF | View/Open |
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