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http://nopr.niscpr.res.in/handle/123456789/15382| Title: | Structural and immunological characterisation of partially reduced chicken riboflavin carrier protein |
| Authors: | Deshmukh, Anuradha Gani, M Natraj, Usha |
| Issue Date: | Jun-2000 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | To improve the accessibility of highly folded chicken riboflavin carrier protein (cRCP) towards proteolytic enzymes, partial disulphide reduction of the protein was carried out. The structural and immunological alteration following reduction and alkylation of two out of the total nine disulphide bonds (2S-CMRCP) of cRCP was studied. The ability of the protein to interact with riboflavin was completely abolished with no change in its far UV, CD spectra. Monoclonal antibodies directed towards the assembled epitopes of cRCP also interacted with 2S-CMRCP to a similar extent. However, sandwich assays with the mAbs revealed that the topography of the epitopes of 2S-CMRCP is subtly altered. 2S-CMRCP was more accessible to chymotrypsin and two peptides were obtained by chymotryptic digestion of 2S-CMRCP. |
| Page(s): | 166-170 |
| ISSN: | 0975-0959 (Online); 0301-1208 (Print) |
| Appears in Collections: | IJBB Vol.37(3) [June 2000] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJBB 37(3) 166-170.pdf | 1.2 MB | Adobe PDF | View/Open |
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