<span style="font-size:12.0pt;font-family: " times="" new="" roman";mso-fareast-font-family:"times="" roman";mso-ansi-language:="" en-in;mso-fareast-language:en-in;mso-bidi-language:ar-sa"="" lang="EN-IN">Analysis of the activity of promoters from two photosynthesis<b>-</b>related gene <i>psa</i><span style="mso-bidi-font-style:italic">F<i> </i>and <i>pet</i><span style="mso-bidi-font-style:italic">H<i> </i>of spinach in a monocot plant, rice </span></span></span>

Abstract

<span style="font-size:12.0pt;font-family: " times="" new="" roman";mso-fareast-font-family:"times="" roman";mso-ansi-language:="" en-in;mso-fareast-language:en-in;mso-bidi-language:ar-sa"="" lang="EN-IN">The subunit III of phtosystem I and ferredoxin-NADP⁺-oxidoreductase are encoded by nuclear genes, namely <i style="mso-bidi-font-style:normal">psa</i>F and <i>pet</i><span style="mso-bidi-font-style:italic">H<i>. </i>The activity of their promoters from spinach has been evaluated in transgenic tobacco earlier. Evaluation of the activity of these <i>Dicotyledoneae-</i><span style="mso-bidi-font-style:italic">specific<i> </i>promoters has been carried out in a monocot system (i.e. rice) by transient gene expression system, based on electroporation-mediated gene delivery into protoplasts from leaves and roots. It has been found that various promoter deletions show higher activity in leaf protoplasts and elements for quantitative response are widely distributed. Transgenic rice has also been produced with a <i>pet</i><span style="mso-bidi-font-style: italic">H<i> </i>promoter and <i style="mso-bidi-font-style:normal">gus<span style="mso-bidi-font-style:italic"> </span></i>reporter gene construct. Although <i>pet</i><span style="mso-bidi-font-style:italic">H<i> </i>promoter is a weak promoter in comparison to the <i style="mso-bidi-font-style: normal"><span style="font-size:12.0pt;font-family:" times="" new="" roman";="" mso-fareast-font-family:hiddenhorzocr;mso-ansi-language:en-in;mso-fareast-language:="" en-in;mso-bidi-language:ar-sa"="" lang="EN-IN">35S</span></i><span style="font-size: 12.0pt;font-family:" times="" new="" roman";mso-fareast-font-family:"times="" roman";="" mso-ansi-language:en-in;mso-fareast-language:en-in;mso-bidi-language:ar-sa"="" lang="EN-IN"> promoter, it expresses well in green tissues and could be useful for plant genetic engineering.</span></span></span></span></span></span>