n-Propanol as a substrate for assaying the ligninperoxidase activity of Phanerochaete chrysoporium

Shanmugam, V; Kumari, Meera; Yadav, K D S

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Please use this identifier to cite or link to this item: http://nopr.niscpr.res.in/handle/123456789/15411

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DC Field	Value	Language
dc.contributor.author	Shanmugam, V	-
dc.contributor.author	Kumari, Meera	-
dc.contributor.author	Yadav, K D S	-
dc.date.accessioned	2012-12-30T20:24:49Z	-
dc.date.available	2012-12-30T20:24:49Z	-
dc.date.issued	1999-02	-
dc.identifier.issn	0975-0959 (Online); 0301-1208 (Print)	-
dc.identifier.uri	http://hdl.handle.net/123456789/15411	-
dc.description	39-43	en_US
dc.description.abstract	The steady state kinetics of ligninperoxidase catalysed reaction using n-propanol as the organic substrate and monitoring the formation of propanaldehyde at λ = 300 nm spectropbotometerically as functions of different reaction parameters has been studied. It has been concluded that n-propanol can be used as a substrate for analysing the activity of ligninperoxidase. The turnover number of ligninperoxidase of Phanerochaete chrysosporium using n-propanol as substrate has been found to be higher approximately by a factor of 10³ as compared to that using veratryl alcohol as the substrate. The method works in assaying the activity of Iigninperoxidase produced by Aspergillus fumigatus indicating that it can be used for assaying the ligninperoxidase activities produced by other microorganisms also and is not limited to assaying the ligninase activity produced by Phanerochaete chrysosporium alone. Under identical experimental conditions, horseradish peroxidase does not show peroxidase activity using n-propanol as substrate indicating that the method does not interfere with the activities of other peroxidases.	en_US
dc.language.iso	en_US	en_US
dc.publisher	NISCAIR-CSIR, India	en_US
dc.rights	CC Attribution-Noncommercial-No Derivative Works 2.5 India	en_US
dc.source	IJBB Vol.36(1) [February 1999]	en_US
dc.title	n-Propanol as a substrate for assaying the ligninperoxidase activity of Phanerochaete chrysoporium	en_US
dc.type	Article	en_US
Appears in Collections:	IJBB Vol.36(1) [February 1999]

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